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real time pcr system  (Roche)


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    Structured Review

    Roche real time pcr system
    Real Time Pcr System, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lightcycler+480+real+time+pcr+system/LightCycler+480+System/pmc12996981-51-18-21
    Average 99 stars, based on 6 article reviews
    real time pcr system - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    cDNA Synthesis:

    Article Title: Pharmacological potential of the histone demethylase inhibitor GSK-J4 in mitigating NF-κB-mediated inflammatory cascades associated with autoimmune disorders.
    Article Snippet: Relative gene expression analysis The Direct-zol RNA Miniprep Kit (ZYMO, USA) was used to isolate total RNA from different cell groups. .. Using the COSMO cDNA synthesis kit (Willowfort, UK), reverse transcription was accomplished. qRT-PCR assays were performed on the LightCycler® 480 real-time PCR system (Roche, Germany) utilizing the HERA SYBR® Green qPCR Kit (Willowfort, UK). ..

    Article Title: ATP6V1B2 Regulating Autophagy Flux To Affect Abnormal Auditory Development.
    Article Snippet: To investigate the expression changes of ATP6V1B2 downregulation in Dominant Deafness-Onychodystrophy syndrome (DDOD syndrome), its impact on auditory function, and the underlying molecular mechanisms, thereby clarifying the role of ATP6V1B2 in maintaining inner ear auditory function.. RNA interference was used to downregulate ATP6V1B2 expression in HEI-OC1 cells.. A DDOD syndrome mouse model was established via cochlear microinjection of ATP6V1B2-specific morpholino, while control mice injected with a scramble morpholino.

    Reverse Transcription:

    Article Title: Pharmacological potential of the histone demethylase inhibitor GSK-J4 in mitigating NF-κB-mediated inflammatory cascades associated with autoimmune disorders.
    Article Snippet: Relative gene expression analysis The Direct-zol RNA Miniprep Kit (ZYMO, USA) was used to isolate total RNA from different cell groups. .. Using the COSMO cDNA synthesis kit (Willowfort, UK), reverse transcription was accomplished. qRT-PCR assays were performed on the LightCycler® 480 real-time PCR system (Roche, Germany) utilizing the HERA SYBR® Green qPCR Kit (Willowfort, UK). ..

    Article Title: m6A-modified circFOXA1 promotes tumor immune evasion via enhancing PD-L1 deubiquitination in triple-negative breast cancer.
    Article Snippet: .. After digestion, RNA was reverse-transcribed into complementary DNA (cDNA), and the expression levels of circFOXA1 and linear FOXA1 were detected by qRT-PCR to evaluate the resistance of circFOXA1 to RNase R digestion. qRT-PCR Total RNA was extracted from TNBC cells and clinical tissues using TRIzolTM Reagent according to the manufacturer’s instructions. qRT-PCR was performed on a LightCycler 480 Real-Time PCR System (Roche, Basel, Switzerland) using the SYBR® Premix Ex TaqTM II Kit (TaKaRa). ..

    Quantitative RT-PCR:

    Article Title: Pharmacological potential of the histone demethylase inhibitor GSK-J4 in mitigating NF-κB-mediated inflammatory cascades associated with autoimmune disorders.
    Article Snippet: Relative gene expression analysis The Direct-zol RNA Miniprep Kit (ZYMO, USA) was used to isolate total RNA from different cell groups. .. Using the COSMO cDNA synthesis kit (Willowfort, UK), reverse transcription was accomplished. qRT-PCR assays were performed on the LightCycler® 480 real-time PCR system (Roche, Germany) utilizing the HERA SYBR® Green qPCR Kit (Willowfort, UK). ..

    Article Title: Opposing behavioral roles for a single gene in a species with a supergene polymorphism
    Article Snippet: .. Expression of the VIP gene was measured using RT-qPCR performed using a Roche LightCycler 480 Real-Time PCR System. ..

    Article Title: m6A-modified circFOXA1 promotes tumor immune evasion via enhancing PD-L1 deubiquitination in triple-negative breast cancer.
    Article Snippet: .. After digestion, RNA was reverse-transcribed into complementary DNA (cDNA), and the expression levels of circFOXA1 and linear FOXA1 were detected by qRT-PCR to evaluate the resistance of circFOXA1 to RNase R digestion. qRT-PCR Total RNA was extracted from TNBC cells and clinical tissues using TRIzolTM Reagent according to the manufacturer’s instructions. qRT-PCR was performed on a LightCycler 480 Real-Time PCR System (Roche, Basel, Switzerland) using the SYBR® Premix Ex TaqTM II Kit (TaKaRa). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Pharmacological potential of the histone demethylase inhibitor GSK-J4 in mitigating NF-κB-mediated inflammatory cascades associated with autoimmune disorders.
    Article Snippet: Relative gene expression analysis The Direct-zol RNA Miniprep Kit (ZYMO, USA) was used to isolate total RNA from different cell groups. .. Using the COSMO cDNA synthesis kit (Willowfort, UK), reverse transcription was accomplished. qRT-PCR assays were performed on the LightCycler® 480 real-time PCR system (Roche, Germany) utilizing the HERA SYBR® Green qPCR Kit (Willowfort, UK). ..

    Article Title: Enterohemorrhagic Escherichia coli O157:H7 responds to norepinephrine gradients by tRNA reprogramming and codon-biased translation of virulence genes.
    Article Snippet: .. Constructed libraries were submitted to the MIT BioMicro Center, where they underwent quality assessment using the AATI Fragment Analyzer (Agilent) and were quantified by the LightCycler 480 Real-Time PCR System (Roche). .. Libraries were sequenced on the Illumina MiSeq platform using the v3 reagent kit (Illumina) to obtain 75-bp paired-end reads.

    Article Title: Gestational diabetes alters subgingival pathobiont composition
    Article Snippet: Thermo Fisher Scientific, NanoDrop 2000 Spectrophotometer, Waltham, MA, USA. .. Roche Diagnostics, LightCycler 480 Real-Time PCR System, Basel, Switzerland. .. IBM Corp., IBM SPSS Statistics for Windows, Version 23.0, Armonk, NY, USA.

    Article Title: Gestational diabetes alters subgingival pathobiont composition.
    Article Snippet: ‡ Thermo Fisher Scientific, NanoDrop 2000 Spectrophotometer, Waltham, MA, USA. .. § Roche Diagnostics, LightCycler 480 Real-Time PCR System, Basel, Switzerland. .. ‖ IBM Corp., IBM SPSS Statistics for Windows, Version 23.0, Armonk, NY, USA.

    Article Title: ATP6V1B2 Regulating Autophagy Flux To Affect Abnormal Auditory Development.
    Article Snippet: To investigate the expression changes of ATP6V1B2 downregulation in Dominant Deafness-Onychodystrophy syndrome (DDOD syndrome), its impact on auditory function, and the underlying molecular mechanisms, thereby clarifying the role of ATP6V1B2 in maintaining inner ear auditory function.. RNA interference was used to downregulate ATP6V1B2 expression in HEI-OC1 cells.. A DDOD syndrome mouse model was established via cochlear microinjection of ATP6V1B2-specific morpholino, while control mice injected with a scramble morpholino.

    Article Title: Opposing behavioral roles for a single gene in a species with a supergene polymorphism
    Article Snippet: .. Expression of the VIP gene was measured using RT-qPCR performed using a Roche LightCycler 480 Real-Time PCR System. ..

    Article Title: m6A-modified circFOXA1 promotes tumor immune evasion via enhancing PD-L1 deubiquitination in triple-negative breast cancer.
    Article Snippet: .. After digestion, RNA was reverse-transcribed into complementary DNA (cDNA), and the expression levels of circFOXA1 and linear FOXA1 were detected by qRT-PCR to evaluate the resistance of circFOXA1 to RNase R digestion. qRT-PCR Total RNA was extracted from TNBC cells and clinical tissues using TRIzolTM Reagent according to the manufacturer’s instructions. qRT-PCR was performed on a LightCycler 480 Real-Time PCR System (Roche, Basel, Switzerland) using the SYBR® Premix Ex TaqTM II Kit (TaKaRa). ..

    SYBR Green Assay:

    Article Title: Pharmacological potential of the histone demethylase inhibitor GSK-J4 in mitigating NF-κB-mediated inflammatory cascades associated with autoimmune disorders.
    Article Snippet: Relative gene expression analysis The Direct-zol RNA Miniprep Kit (ZYMO, USA) was used to isolate total RNA from different cell groups. .. Using the COSMO cDNA synthesis kit (Willowfort, UK), reverse transcription was accomplished. qRT-PCR assays were performed on the LightCycler® 480 real-time PCR system (Roche, Germany) utilizing the HERA SYBR® Green qPCR Kit (Willowfort, UK). ..

    Article Title: ATP6V1B2 Regulating Autophagy Flux To Affect Abnormal Auditory Development.
    Article Snippet: To investigate the expression changes of ATP6V1B2 downregulation in Dominant Deafness-Onychodystrophy syndrome (DDOD syndrome), its impact on auditory function, and the underlying molecular mechanisms, thereby clarifying the role of ATP6V1B2 in maintaining inner ear auditory function.. RNA interference was used to downregulate ATP6V1B2 expression in HEI-OC1 cells.. A DDOD syndrome mouse model was established via cochlear microinjection of ATP6V1B2-specific morpholino, while control mice injected with a scramble morpholino.

    Construct:

    Article Title: Enterohemorrhagic Escherichia coli O157:H7 responds to norepinephrine gradients by tRNA reprogramming and codon-biased translation of virulence genes.
    Article Snippet: .. Constructed libraries were submitted to the MIT BioMicro Center, where they underwent quality assessment using the AATI Fragment Analyzer (Agilent) and were quantified by the LightCycler 480 Real-Time PCR System (Roche). .. Libraries were sequenced on the Illumina MiSeq platform using the v3 reagent kit (Illumina) to obtain 75-bp paired-end reads.

    Synthesized:

    Article Title: ATP6V1B2 Regulating Autophagy Flux To Affect Abnormal Auditory Development.
    Article Snippet: To investigate the expression changes of ATP6V1B2 downregulation in Dominant Deafness-Onychodystrophy syndrome (DDOD syndrome), its impact on auditory function, and the underlying molecular mechanisms, thereby clarifying the role of ATP6V1B2 in maintaining inner ear auditory function.. RNA interference was used to downregulate ATP6V1B2 expression in HEI-OC1 cells.. A DDOD syndrome mouse model was established via cochlear microinjection of ATP6V1B2-specific morpholino, while control mice injected with a scramble morpholino.

    Expressing:

    Article Title: Opposing behavioral roles for a single gene in a species with a supergene polymorphism
    Article Snippet: .. Expression of the VIP gene was measured using RT-qPCR performed using a Roche LightCycler 480 Real-Time PCR System. ..

    Article Title: m6A-modified circFOXA1 promotes tumor immune evasion via enhancing PD-L1 deubiquitination in triple-negative breast cancer.
    Article Snippet: .. After digestion, RNA was reverse-transcribed into complementary DNA (cDNA), and the expression levels of circFOXA1 and linear FOXA1 were detected by qRT-PCR to evaluate the resistance of circFOXA1 to RNase R digestion. qRT-PCR Total RNA was extracted from TNBC cells and clinical tissues using TRIzolTM Reagent according to the manufacturer’s instructions. qRT-PCR was performed on a LightCycler 480 Real-Time PCR System (Roche, Basel, Switzerland) using the SYBR® Premix Ex TaqTM II Kit (TaKaRa). ..



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